Trypan Blue Exclusion Assay Verifies in Vitro Cytotoxicity of New Cis-Platinum (II) Complex in Human Cells
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چکیده
منابع مشابه
Trypan blue exclusion assay by flow cytometry
Dye exclusion tests are used to determine the number of live and dead cells. These assays are based on the principle that intact plasma membranes in live cells exclude specific dyes, whereas dead cells do not. Although widely used, the trypan blue (TB) exclusion assay has limitations. The dye can be incorporated by live cells after a short exposure time, and personal reliability, related to the...
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Hyperthermic enhancement of cis-diammine-l,l-cyclobutane dicarboxylate platinum(II) (carboplatin) cytotoxicity was studied in vitro in .IM, a human acute lyntphoblastic leukemia cell line. Corrected for direct heat toxicity, hyperthermia enhanced carboplatin killing at the clinically relevant temperatures of 40.5°and 41.8°C.The thermal enhancement ratios at these temperatures were 1.89 and 33...
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ژورنال
عنوان ژورنال: Baghdad Science Journal
سال: 2019
ISSN: 2411-7986,2078-8665
DOI: 10.21123/bsj.2019.16.3.0555